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KMID : 1094720140190020298
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Biotechnology and Bioprocess Engineering
2014 Volume.19 No. 2 p.298 ~ p.303
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Synthesis and purification of kaempferol by enzymatic hydrolysis of tea seed extract
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Lee Seul-Bi
Chung Dae-Won
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Abstract
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We have made an in-depth study on elucidating selective hydrolysis reaction by enzyme on two kinds of flavonol triglycosides, camelliaside A (CamA) and camelliaside B (CamB). In this paper, we employ five kinds of commercial enzyme complexes and report their effect on the hydrolysis reaction of CamA and CamB. Ultraflo, Celluclast and Shearzyme selectively hydrolyze the xylosyl moiety of CamB, producing primarily kaempferol diglycoside, while Dextrozyme yielding monoglycoside. Whereas, Viscozyme transform CamA and CamB into kaempferol (KR) by non-specific hydrolysis. We recover KR with 95.4% purity from a scale-up reaction with Viscozyme followed by recrystallization of crude KR.
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KEYWORD
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tea seed extract, kaempferol, flavonol triglycosides, viscozyme, enzymatic hydrolysis
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